Capillary electrophoresis analyzer Capel-105M

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About
Capel-105M is a benchtop capillary electrophoresis system designed for routine and advanced analyses in food, environmental, pharmaceutical, chemical, biochemical and forensic laboratories. The system combines flexible injection modes, precise capillary thermostating and a UV spectrophotometric detector to support quantitative and qualitative workflows.

Principle of operation
Capillary electrophoresis separates ionic components of aqueous samples by their differential migration in a narrow fused-silica capillary under an electric field. Separated analytes are monitored and quantified using a UV spectrophotometric detector appropriate for absorbance-based detection in the 190–400 nm range.

Features and benefits

• Spectral scanning for peak identification and method development
• Wide range of controlled injection pressures for analysis of low- and high-viscosity samples
• Reverse sample injection under vacuum for ultra-short runs (sub-1 minute) and sample stacking to lower limits of detection

Precise capillary thermostating via circulating liquid coolant (water) with ±0.1 °C stability:

• Supports a broader range of buffers and improves separation efficiency

Elforun software package for instrument control and data processing:

• Integrated control, acquisition and processing with real-time electropherogram display
• Programmable sequences including dynamic changes of wavelength, pressure and voltage
• Custom report generation and data export to spreadsheets and databases; GLP/GALP compliance

Capillary cassette design:

• Quick capillary replacement in minutes
• Optimized optical layout for improved detection limits

Analytical characteristics
Detectors
Spectrophotometric detector covering 190–400 nm.

Light source
Deuterium lamp.

Vials
Standard Eppendorf-type 1.5 ml vials; minimal sample volume 100 µl.

Injection modes
Pressure, vacuum or electrokinetic injection. Controlled pressure profile from −99 mbar to +99 mbar. Electrokinetic injection at ±1–25 kV with programmable ramping. Injection time, pressure and voltage are programmable.

Analysis
Voltage range −25 kV to +25 kV in 1 kV steps with electronic polarity switching. Current 0–200 µA.

Elforun software features

• Instrument control, data acquisition and processing in a single environment
• Real-time electropherogram visualization and overlay mode
• Data processing with basic mathematical operations and extensive customization
• Parameter computation, customized reports, and data exchange with external programs
• GLP and GALP support

Equipment and options

• Capillary electrophoresis system CAPEL-105M
• Spare capillary cassettes
• Elforun software package
• Analysis kits on request; compatible with most CE kits from other manufacturers

Warranty
All Lumex instruments are covered by a 12-month warranty.

Services
On-site installation by company service engineers and customer-specific operator training are available.

Technical specifications

• Detector: Spectrophotometer, 190–400 nm
• Analysis: Constant voltage ±1–25 kV in 1 kV steps; electronic polarity switch; current 0–200 µA; pressure ±1–99 mbar; programmable changes of wavelength, pressure and voltage during runs
• Injection: Electrokinetic, pressure, vacuum
• Sampler: Autosampler for 10 inlet and 10 outlet vials
• Rinsing: Pressure ~1000 or 2000 mbar
• Capillary: Length 42–100 cm; internal diameter 50, 75 µm
• Capillary temperature control: From 10 °C below ambient (not below +5 °C absolute) to +50 °C
• Controlled parameters: Wavelength, pressure, temperature, voltage
• Power requirements: 110/220 Vac, 50/60 Hz
• Power consumption: 200 W
• Dimensions: 500×410×365 mm
• Weight: 25 kg