AboutCapel-205 implements capillary electrophoresis (CE), a high-resolution separation technique for simultaneous determination of multiple analytes in aqueous and aqueous–organic samples. Short run times and high separation efficiency make the system suitable for environmental testing, food and beverage analysis, chemical and pharmaceutical laboratories, biochemistry and research.
Main advantages- Versatile CE modes for a wide range of applications (CZE, CGE, CIEF and others)
- Single instrument for diverse analytes in aqueous–organic matrices
- Short analysis time and large autosampler capacity improve laboratory throughput
- No external peripheral equipment required for basic operation
- Low reagent and sample consumption; use of standard consumables reduces operating costs
- Sealed vials with automatic opening prevent evaporation and contamination in the autosampler
- Elforun software compliant with 21 CFR part 11
- Optional streaming potential control for improved migration-time repeatability
Principle of operationCE separates sample components by differential migration in a narrow fused-silica capillary under an electric field. Detection is performed by a sensitive optical system based on direct or indirect UV absorbance. The system supports main CE modes including capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF).
ApplicationsEnvironmental analysis- Natural, drinking and wastewater: inorganic and organic ions (Br−, Cl−, F−, I−, NO2−, NO3−, PO43−, SO42−, acetate, NH4+, Ba2+, Ca2+, K+, Mg2+, Na+ and others)
- Soils, sludge and sediments: inorganic and organic anions and cations
Food testing- Bottled water: inorganic and organic anions and cations
- Non-alcoholic and alcoholic beverages: sugars, sweeteners, preservatives, organic acids, synthetic dyes, antioxidants, inorganic ions; aromatic aldehydes in spirits
- Foodstuffs, tea and coffee: sugars, amino acids, flavor enhancers, caffeine, theobromine, inorganic ions
Animal feed & Veterinary- Feed and raw materials: amino acids, organic acids, cations and anions, vitamins
- Veterinary drugs: antibiotics and antiprotozoal agents
Chemical industry- Fertilizer analysis: nitrogen forms, sulfur, phosphorus, K+, Na+, Mg2+, Ca2+, Cl−, F−
- Process monitoring and raw material control
Pharma/Biopharma- Quality control and characterization of therapeutic recombinant proteins
- Protein and enantiomer separations; pharmacokinetic studies
Equipment and options- Capillary electrophoresis system CAPEL-205
- Spare capillary cassettes and capillaries (40–120 cm; ID 50 or 75 μm)
- Elforun control software (21 CFR part 11 compliant)
- Analysis kits on request; compatibility with many CE kits from other manufacturers
- Optional streaming potential control facility
WarrantyLumex Instruments’ CE systems are covered by a full 1-year warranty.
ServicesInstallation can be performed on-site by service engineers or certified distributor engineers. User training tailored to laboratory needs is available.
Specifications- Detector: spectrophotometric
- Light source: deuterium lamp
- Detection wavelength: 190–400 nm
- Analysis: constant voltage ±1–25 (30)* kV in 1 kV steps; electronic polarity switching; current 0–300 μA; pressure up to 100 mbar; programmable wavelength, pressure and voltage changes during run
- Injection: by voltage ±1–25 (30)* kV in 1 kV steps; by pressure −100 to 100 mbar in 1 mbar steps
- Rinsing: by pressure 500–2000 mbar in 1 mbar steps
- Capillary: length 40–120 cm; ID 50 μm or 75 μm
- Capillary temperature control: ambient −10 °C (not below +5 °C) to +50 °C
- Sampler: autosampler for 59 vials (standard microcentrifuge-type 1.5 mL) with sealed vials and automatic opening
- Controlled parameters: wavelength, pressure, temperature, voltage
- Control: Elforun software (21 CFR part 11 compliant)
- Streaming potential control: optional
- Power supply: 110–240 VAC, 50/60 Hz; consumption 170 W
- Dimensions: 470×530×410 mm; Weight: 30 kg